Title: |
Title:
Counteracting Antibiotic Resistances, Small Non-Coding RNA Induced Gene Silencing of Antibiotic Resistance Genes in E.Coli DH5α
|
Authors: |
Authors:
Kathe, Nina, Alte Kantonsschule Aarau, Aarau, SWITZERLAND
|
Contributors: |
Contributors:
Editor: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887HostingInstitution: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887 |
Pages: |
Pages:
14
|
Languages: |
Languages:
en
|
DOI: |
DOI:
10.7795/320.201904
|
Resource Type: |
Resource Type:
Text / Article
|
Publisher: |
Publisher:
Physikalisch-Technische Bundesanstalt (PTB)
|
Rights: |
Rights:
Vervielfältigung nur zum eigenen persönlichen Gebrauch.
|
Dates: |
Dates:
Available: 2019-05-15 Accepted: 2017-10-03 Submitted: 2017-06-18 |
File: |
File:
Download File
(application/pdf)
7.58 MB (7943162 Bytes)
MD5 Checksum: 62d3cf1ab65636bc4214e43e7f8c0e78 SHA256 Checksum: a2c4d39e10f9749f0d2f173bacc8a640d0cd02ccf9977036c9767a58e5365635 |
Abstract: |
Abstract:
The aim was to determine whether small non-coding RNAs (sRNAs) are able to silence antibiotic resistance genes in bacteria. If so, the bacteria would lose their resistance. Therefore, such sRNAs were designed against GFP gene, tetracycline (Tc) and chloramphenicol (Cm) resistance gene. In presence of these sRNAs and the antibiotic, only few Tc resistant bacteria had grown, thus the Tc resistance gene was successfully silenced. In contrast, the number of Cm resistant bacteria remained unchanged and GFP-expressing bacteria continued to glow green, hence those genes were not silenced.
|
Series Information: |
Series Information:
Junge Wissenschaft. Paper 04/2019
|
Other: |
Other:
In der Jungen Wissenschaft werden Forschungsarbeiten von Schüler/innen, die selbstständig, z.B. in einer Schule oder einem Schülerforschungszentrum, durchgeführt wurden, veröffentlicht.
|
Citation: |
Citation:
Kathe, Nina. Counteracting Antibiotic Resistances - Small Non-Coding RNA Induced Gene Silencing of Antibiotic Resistance Genes in E.Coli DH5α. Physikalisch-Technische Bundesanstalt (PTB), 2019. Verfügbar unter: https://doi.org/10.7795/320.201904
|

-OAR