Title: Counteracting Antibiotic Resistances, Small Non-Coding RNA Induced Gene Silencing of Antibiotic Resistance Genes in E.Coli DH5α
Authors: Kathe, Nina, Alte Kantonsschule Aarau, Aarau, SWITZERLAND
Contributors: Editor: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887
HostingInstitution: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887
Pages:14
Language:en
DOI:10.7795/320.201904
Resource Type: Text / Article
Publisher: Physikalisch-Technische Bundesanstalt (PTB)
Rights: Vervielfältigung nur zum eigenen persönlichen Gebrauch.
Dates: Available: 2019-05-15
Accepted: 2017-10-03
Submitted: 2017-06-18
File: Download File (application/pdf) 7.58 MB (7943162 Bytes)
MD5 Checksum: 62d3cf1ab65636bc4214e43e7f8c0e78
SHA256 Checksum: a2c4d39e10f9749f0d2f173bacc8a640d0cd02ccf9977036c9767a58e5365635
Abstract: The aim was to determine whether small non-coding RNAs (sRNAs) are able to silence antibiotic resistance genes in bacteria. If so, the bacteria would lose their resistance. Therefore, such sRNAs were designed against GFP gene, tetracycline (Tc) and chloramphenicol (Cm) resistance gene. In presence of these sRNAs and the antibiotic, only few Tc resistant bacteria had grown, thus the Tc resistance gene was successfully silenced. In contrast, the number of Cm resistant bacteria remained unchanged and GFP-expressing bacteria continued to glow green, hence those genes were not silenced.
Series Information: Junge Wissenschaft. Paper 04/2019
Other: In der Jungen Wissenschaft werden Forschungsarbeiten von Schüler/innen, die selbstständig, z.B. in einer Schule oder einem Schülerforschungszentrum, durchgeführt wurden, veröffentlicht.
Citation: Kathe, Nina. Counteracting Antibiotic Resistances - Small Non-Coding RNA Induced Gene Silencing of Antibiotic Resistance Genes in E.Coli DH5α. Physikalisch-Technische Bundesanstalt (PTB), 2019. Verfügbar unter: https://doi.org/10.7795/320.201904