Title: Counteracting Antibiotic Resistances, Small non-coding RNA induced gene silencing of antibiotic resistance genes in E. coli DH5α
Authors: Kathe, Nina, Alte Kantonsschule Aarau, Aarau, SCHWEIZ
Contributors: Editor: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887
HostingInstitution: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887
Pages:14
Language:en
DOI:10.7795/320.201904
Resource Type: Text / Article
Publisher: Physikalisch-Technische Bundesanstalt (PTB)
Rights: Vervielfältigung nur zum eigenen persönlichen Gebrauch.
Dates: Available: 2019-10-23
Accepted: 2017-06-18
Submitted: 2017-10-03
File: Download File (application/pdf) 7.66 MB (8032510 Bytes)
MD5 Checksum: 70141262b4ea488fe488a13203206ab5
SHA256 Checksum: 7d94f7e2a1990fe1ddc8ae408bb9814279fd2d971575d6c31f0dd331ece8d0ad
Keywords: antibiotics ; tetracycline ; chloramphenicol ; antibiotic resistance ; bacteria ; E. coli DH5alpha ; sRNA ; RNA interference ; GFP ; vector cloning
Abstract: The aim was to determine whether small non-coding RNAs (sRNAs) are able to silence antibiotic resistance genes in bacteria. If so, the bacteria would lose their resistance. Therefore, such sRNAs were designed against GFP gene, tetracycline (Tc) and chloramphenicol (Cm) resistance gene. In presence of these sRNAs and the antibiotic, only few Tc resistant bacteria had grown, thus the Tc resistance gene was successfully silenced. In contrast, the number of Cm resistant bacteria remained unchanged and GFP-expressing bacteria continued to glow green, hence those genes were not silenced.
Series Information: Junge Wissenschaft. Paper 04/2019
Other: In der Jungen Wissenschaft werden Forschungsarbeiten von Schüler/innen, die selbstständig, z.B. in einer Schule oder einem Schülerforschungszentrum, durchgeführt wurden, veröffentlicht.
Citation: Kathe, Nina. Counteracting Antibiotic Resistances - Small non-coding RNA induced gene silencing of antibiotic resistance genes in E. coli DH5α. Physikalisch-Technische Bundesanstalt (PTB), 2019. Verfügbar unter: https://doi.org/10.7795/320.201904