Title: Final report on the project Volume Determination in the Microlitre Range
Authors: Wolf, Henning, Physikalisch-Technische Bundesanstalt (PTB), Fachbereich 3.1, Allgemeine und Anorganische Chemie
Contributors: HostingInstitution: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887
Resource Type: Text / Report
Publisher: Physikalisch-Technische Bundesanstalt (PTB)
Rights: Download for personal/private use only, if your national copyright law allows this kind of use.
Relationships: IsPartOf: ISSN 1434-2391
IsIdenticalTo: ISBN 978-3-95606-582-8
Dates: Available: 2021-09-06
Created: 2021-03
File: Download File (application/pdf) 2.14 MB (2246827 Bytes)
MD5 Checksum: 36873195061de977619a36780c525eb3
SHA256 Checksum: 4c877f98fecdc4bfab70a7612cad73087c185827ca32070068cdaee7173438a6
Keywords: gravimetric calibration of volumetric dosing devices ; photometric calibration of volumetric dosing devices ; piston-stroke pipettes ; microlitre syringes ; evaporation effects in gravimetric calibration ; density of human serum ; dosing of different liquids ; dosing of human serum
Abstract: This report describes investigations of the dosing behaviour of piston-stroke pipettes and microlitre syringes when dosing different liquids. The dosed volumes were in the range between 0.2 µl and 50 µl, they were measured with a gravimetric measuring apparatus. The liquids used were water, ethanol and nonane, with a special focus on investigating the dosing behaviour of human serum. In the course of the investigations, the gravimetric measuring method was also compared experimentally with the photometric method. In order to enable gravimetric calibrations of volume dosing devices with human serum in analytical laboratories, a simple method for determining the density of human serum from the analytically determined saline and protein concentrations in the serum was developed.
Citation: Wolf, Henning. Final report on the project Volume Determination in the Microlitre Range, 2021. Physikalisch-Technische Bundesanstalt (PTB). DOI: https://doi.org/10.7795/110.20210906