Title:
Title:
Flow Cytometry Pulse Shape Data and their derived quantities
Authors:
Authors:
Putz, Alexander, Physikalisch-Technische Bundesanstalt (PTB), Fachbereich 8.3, Biomedizinische Optik, ORCID: 0000-0002-6497-2672
Gienger, Jonas, Physikalisch-Technische Bundesanstalt (PTB), Fachbereich 8.3, Biomedizinische Optik, ORCID: 0000-0003-1080-8874
Hussels, Martin, Physikalisch-Technische Bundesanstalt (PTB), Fachbereich 8.3, Biomedizinische Optik, ORCID: 0000-0002-9538-6107
Contributors:
Contributors:
HostingInstitution: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887
Languages:
Languages:
en
DOI:
DOI:
10.7795/720.20250812
Resource Type:
Resource Type:
PTB: Measurement data, DINI: ResearchData, DataCite: Dataset
Publisher:
Publisher:
Physikalisch-Technische Bundesanstalt (PTB)
Rights:
Rights:
https://creativecommons.org/licenses/by/4.0/
CC-BY 4.0 International
Dates:
Dates:
Available: 2025-08-12
File:
File:
Download File (application/zip) 490.3 MB
MD5 Checksum: ceb7cd65c01b76a7312cc37ddd2ebf0b
SHA256 Checksum: a96bd99a1d6f216623487d255d2d8f7137c11ca990d4e2d2553f0817ee7f0f12
Keywords:
Keywords:
light scattering ; flow cytometry ; pulse shape analysis ; light scattering modeling ; label-free
Abstract:
Abstract:
These files contain the measured pulse data used in the paper. Originally the data was saved in tdms and fcs files, but was converted to hdf5 and csv files for better accessibility. Channel names are forward scattering (fsc) and side scattering (ssc). The h5 file was then postprocessed and the resulting quantities saved in a csv file.
Table of Contents:
Table of Contents:
Folder raw data:
PS1540nm.h5:
research object: polystyrene particles (PS)
method: Flow Cytometer
format: h5
recommended software: python nptdms package
parameters: size 1540nm, laserwavelength 488nm, monodisperse

PS 1mu+5mu 1.03microl_per_second.h5:
research object: polystyrene particles
method: Flow Cytometer
format: h5
recommended software: python nptdms package
parameters: 1 µm and 5 µm mixed, 1000 µL of 5 µm PS + 1 µm 1:1000 diluted 1µm PS, laserwavelength 488nm, flowrate 1.03µL/s

PS 1mu+5mu 0.443microl_per_second.h5:
research object: polystyrene particles
method: Flow Cytometer
format: h5
recommended software: python nptdms package
parameters: 1 µm and 5 µm mixed, 1000 µL of 5 µm PS + 1 µm 1:1000 diluted 1µm PS, laserwavelength 488nm, flowrate 0.443µL/s

PS 1mu+5mu 0.177microl_per_second.h5:
research object: polystyrene particles
method: Flow Cytometer
format: h5
recommended software: python nptdms package
parameters: 1 µm and 5 µm mixed, 1000 µL 5 µm PS + 1 µm 1:1000 diluted 1µm PS, laserwavelength 488nm, flowrate 0.177µL/s


Folder postprocessed data:

the data in "raw data" postprocessed regarding describing quantities. See table headers for names.

Authors

Putz, Alexander, Physikalisch-Technische Bundesanstalt (PTB), Fachbereich 8.3, Biomedizinische Optik, ORCID: 0000-0002-6497-2672
Gienger, Jonas, Physikalisch-Technische Bundesanstalt (PTB), Fachbereich 8.3, Biomedizinische Optik, ORCID: 0000-0003-1080-8874
Hussels, Martin, Physikalisch-Technische Bundesanstalt (PTB), Fachbereich 8.3, Biomedizinische Optik, ORCID: 0000-0002-9538-6107

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