Title: Surface-Controlled Adsorption of Plasmonic DNA Origami Nanostructures for Single-Molecule Surface-Enhanced Raman Spectroscopy (SERS)
Authors: Wang, Zunhao, Physikalisch-Technische Bundesanstalt (PTB), Fachbereich 3.1, Allgemeine und Anorganische Chemie, ORCID: 0000-0003-3681-2986
Contributors: HostingInstitution: Physikalisch-Technische Bundesanstalt (PTB), ISNI: 0000 0001 2186 1887
Resource Type: Text / Dissertation
Publisher: Physikalisch-Technische Bundesanstalt (PTB)
Rights: Download for personal/private use only, if your national copyright law allows this kind of use.
Relationships: IsPartOf: ISSN 2941-1297
IsIdenticalTo: ISBN 978-3-944659-31-2
Dates: Available: 2024-02-29
Created: 2024-01
File: Download File (application/pdf) 9.04 MB (9476537 Bytes)
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SHA256 Checksum: a2460d608874ed08dff4543238ba2f09539152ce30d81ec440648b067dfac01e
Keywords surface-enhanced Raman scattering ; DNA origami ; single molecule detection ; controlled physisorption
Abstract: The quantitative detection of biomolecules, such as proteins and peptides, at low concentration levels plays a crucial role in facilitating early diagnosis and monitoring of prevalent diseases. Given the increasing utilization of metrological quantification in life sciences and the mandatory quality assurance requirements in routine laboratories, the need for SI-traceable methods to quantify such biomarkers has become urgent.
This thesis aims to investigate the quantification of substances at the single-molecule level using Surface-enhanced Raman Spectroscopy (SERS), with a specific focus on employing the Polar Surface Array (PSA) as a selective-adsorbing substrate for single-molecule studies. The primary objective is to overcome the challenges associated with nanostructure agglomeration and establish a reliable and efficient SERS-based method for the detection and quantification of individual molecules.
This research presents a pioneering approach for the quantification of single molecules through SERS on an adsorption-controlled and tuneable substrate. By overcoming the challenge of nanostructure agglomeration, the PSA provides a highly effective and reliable platform for quantification of molecules at extremely low concentrations, and studying the over-time dynamics of single plasmonic hot spots. Furthermore, the research sheds light on the principles and benefits of SERS for single-molecule analysis, thus highlighting its specificity and its potential, in combination with the isotope dilution method, for metrological molecular quantification.
Series Information: PTB-Bericht Diss-3
Citation: Wang, Zunhao. Surface-Controlled Adsorption of Plasmonic DNA Origami Nanostructures for Single-Molecule Surface-Enhanced Raman Spectroscopy (SERS), 2024. Physikalisch-Technische Bundesanstalt (PTB). DOI: https://doi.org/10.7795/110.20240208